2017
Soybean SDS management: detection, fungicides and resistance
Contributor/Checkoff:
Category:
Sustainable Production
Keywords:
Crop protectionDiseaseField management
Lead Principal Investigator:
Martin Chilvers, Michigan State University
Co-Principal Investigators:
Project Code:
1706
Contributing Organization (Checkoff):
Institution Funded:
Brief Project Summary:

Seed treatments will be examined for their role in the management of SDS and SCN. Bayer Crop Sciences fungicide seed treatment ILeVO was registered in 2015, and in response a number of companies are testing additional products.
We will test our current diagnostic assays as means of pre-plant SDS risk assessment and work on an assay to quantify Fusarium brasiliense, a newly discovered SDS causing species in Michigan.
We will work with the MSU soybean breeding program to screen mapping populations and AYT lines for SDS resistance in the field. SDS inoculum will be produced in the Chilvers laboratory for the inoculation of SDS field screening trials on the Agronomy farm. We will use our real-time...

Unique Keywords:
#soybean diseases
Information And Results
Project Deliverables

To determine fungicide sensitivity in the lab, the fungicide of interest will be amended to medium in Petri plates, and growth of the Fusarium species will be compared to that of a control. This technique allows for the rapid screening of fungicide efficacy and the monitoring for fungicide resistance.
To identify soybean susceptibility factors, F. virguliforme toxins will be isolated and purified using molecular tools, and exposed to soybean leaf tissues. Soybean varieties that develop symptoms will be assessed for specific susceptibility genes that caused a disease reaction in response to the specific F. virguliforme toxin.
Field trials will utilize standard RCBD layout. Trials will be conducted at the naturally infested Decatur location and on campus in inoculated plots.
Strains of F. brasiliense will be screened for pathogenicity and virulence in growth chamber studies.

Final Project Results

SDS resistance can be classified into foliar resistance to phytotoxins and root resistance to pathogen infection. In our preliminary resistance screen with Dr. Glen Hartman in the University of Illinois, we discovered a soybean differential system that distinguish SDS foliar symptoms into four categories. The discovery implement the resistance of foliar chlorosis and foliar necrosis may be controlled by different genetic loci. In collaboration with Dr. Hartman and Dr. Dechun Wang, we are making crossing between the differential lines and linkage mapping will be applied to understand the genetics of SDS foliar symptoms.
Our risk prediction study found evidence that the SDS pathogen and SCN may act together to make SDS foliar symptoms worse. We are using fluorescent microscopes to determine if these two organisms interact in the absence of soybeans.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.