Over 20 pairs of nodulating (nod+) and non-nodulating (nod-) isolines, derived from elite glyphosate-resistant parents and spanning maturity groups 0 through VIII, underwent yield evaluation across various environments in 2024. Isolines received two nitrogen applications of 150 lbs N/acre, one between the V4-V6 growth stages and another between R2-R4. Numerous homozygous plants consisting of nod- and nod+ NILs were grown as plant rows in 2024 and will be evaluated in yield trials in 2025. Some isoline pairs have been transferred to research groups to conduct additional research.