Genetic material:
According to the aim of this project, the high oleic elite line developed at Michigan State University (MSU) under the soybean breeding program will be bred for resistance against prevalent soybean diseases. For this purpose, high oleic line E18331-34 will be equipped with disease resistance genes against Phytophthora root and stem rot (PRSR) caused by Phytophthora sojae and Phytophthora sansomeana, sudden death syndrome (SDS) caused by Fusarium virguliforme, and Pythium spp. causing damping off.
Deliverable 1: E18331-Rps14-SDS Variety (Germplasm)
The donor line of Rps14 (PI 340029) and SDS (LD01-5907) were planted in the greenhouse at the start of the project along with high oleic line E18331-34 in the greenhouse. The Rps14 donor parent and the SDS donor parent were crossed with the high oleic parent to obtain F1 seeds. The F1 seeds from (PI 340029 X E18331-34) were obtained successfully and were planted again in the greenhouse along with the high oleic parent to make backcrosses. More crosses were made between the SDS resistant parent LD01-5907 and E18331-34 for F1 seeds. After successfully backcrossing F1 (PI 340029 × E18331-34) with the recurrent parent (18331-34) at least five BC1F1 pods were obtained. Successful F1 (E18331-34 × LD01-5907) seeds were also obtained this time. The BC1F1 [E18331-34 × (PI 340029 × E18331-34)] seeds and the F1 (E18331-34 × LD01-5907) seeds were planted again in the greenhouse to make further backcrosses. The F1 seeds and BC1F1 seeds were planted in two batches to obtain the maximum number of successful crosses. Crosses are being made to obtain BC2F1s and BC1s. Plants are expected to mature in around 2-3 months. The BC2F1s and BC1s will be planted again in the greenhouse to start the next round of breeding. Dihybrid crosses will be made in later stages.
Deliverable 2: E18331-Rps14-Pythium Variety (Germplasm)
The donor line of Rps14 (PI 340029) and Pythium(21P059), and the high oleic parent E18331-34 were planted in the greenhouse at the start of the project, and crosses were made between PI 340029 and E18331-34 with two successful crosses obtained. More crosses were made between the Pythium donor parent (21P059) and the high oleic parent to obtain F1 seeds. The F1 seeds (PI 340029 × E18331-34) were planted again in the greenhouse to make backcrosses with the recurrent parent (E18331-34). At least five BC1F1 [E18331-34 × (PI 340029 × E18331-34)] pods were obtained. The Pythium resistant donor line 21P059 was successfully crossed with the HOLL line E18331-34. The F1 (E18331-34 × 21P059) seeds and the BC1F1[E18331-34 × (PI 340029 × E18331-34)] were planted again in the greenhouse to start the next round of backcrossing. The F1 (E18331-34 × 21P059) and the BC1F1[E18331-34 × (PI 340029 × E18331-34)] are being backcrossed with the high oleic parent to obtain BC1F1 seeds and the BC2F1 seeds. The plants will mature in 2-3 months and the seeds will be replanted for the next round of breeding.
Deliverable 3: E18331-Rpsan1-Pythium Variety (Germplasm)
The donor line of Rpsan1 (Colfax) and Pythium (21P059), and the high oleic parent E18331-34 were planted in the greenhouse, and crosses were made between the donor lines and E18331-34. After successfully crossing the donor lines with the high oleic parent, F1 seeds were obtained from both combinations. These F1 seeds, F1 (E18331-34 × Colfax) and F1 (E18331-34 × 21P059), were planted in the greenhouse to make backcrosses with the recurrent parent (high oleic). Both F1s were successfully backcrossed with the high oleic line. Plants will mature in 2-3 months. The BC1s will be harvested and planted for the next cycle of breeding (BC2).