The molecular markers validated during this research project, and the soybean FAD gene mutants identified to confer heat tolerance in this study will serve as the raw material for the breeding soybean for heat-tolerance. Such genotypes are critically needed to sustain soybean production in the face of the predicted rise in the global temperature and to meet the future soybean demand. By identifying markers associated with heat tolerance in soybean, breeders can then use these markers to produce a more adapted soybean for SC. This would give growers in SC the ability to be able to better produce a soybean crop under heat stress, which is oftentimes a big problem for SC growers. In addition, this research will benefit soybean breeders across the rest of the US and around the globe, including the semi-arid tropics of Asia and Africa.