2020
Exploring the Differences in Gene Expression of Drought Sensitive and Drought Tolerant Genotypes Using RNA-Seq
Contributor/Checkoff:
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Sruthi Kutty, Clemson University
Co-Principal Investigators:
Project Code:
Contributing Organization (Checkoff):
Leveraged Funding (Non-Checkoff):
None
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Institution Funded:
Brief Project Summary:

One way to address drought stress is to improve the capabilities of the plant to withstand drought conditions. Plant growth and water use are interrelated processes influenced by genetically controlled morphological and biochemical characters broadly described as water use efficiency components. The objective of this experiment is to conduct a detailed examination of gene expression profiles in two soybean genotypes with contrasting water use efficiency profiles to understand genetic architecture of this trait in lines with elite drought tolerance. Research aims to identify genes that become activated in drought-tolerant soybeans and develop DNA biomarkers that are predictive of drought tolerance.

Key Benefactors:
farmers, agronomists, extension agents, soybean breeders, seed companies

Information And Results
Project Deliverables

Due to the complex nature of the mechanisms associated with drought tolerance in soybean, identifying molecular markers that could be used for selection has proven to be difficult. From this study we hope that by exploring the subtle differences in gene expression of drought sensitive and drought tolerant genotypes will enable us to identify drought related genes and co-expression networks that could be used for selection of traits that are associated with WUE.
This information can shed some light on the mechanisms used by plants to sustain drought and be used to select for soybean varieties with improved water use efficiency. The research proposed has the potential to be particularly relevant to soybean growers in SC, where drought stress tends to be more serve.

We also plan to work with Denise Attaway, PSA Media Relations, to relay information about this project to the public. The target audiences for this communication plan are South Carolina soybean producers, state legislators and the general public. This will involve local, state and regional media with print stories, broadcast pieces (audio for radio stations and video for television stations), as well as digital media and Social Network posts. In addition, results from this study will be presented in reports, field days, conference presentations, and publications in peer-reviewed journals.

Final Project Results

Updated January 4, 2021:

View uploaded report PDF file

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.