2018
Identifying the Perfect Markers and Developing Germplasm for Resistance to Phytophthora Sojae, Pythium spp., and Fusarium Graminearum in Soybean (Year 2 of 1720-172-0125)
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Contributor/Checkoff:
United Soybean Board
Category:
Sustainable Production
Keywords:
(none assigned)
Parent Project:
Identifying the Perfect Markers and Developing Germplasm for Resistance to Phytophthora Sojae, Pythium spp., and Fusarium Graminearum in Soybean (Year 1 of 1720-172-0125)
Lead Principal Investigator:
Anne Dorrance, The Ohio State University
Co-Principal Investigators:
Alison Robertson, Iowa State University
Pengyin Chen, University of Missouri
Henry Nguyen, University of Missouri
Grover Shannon, University of Missouri
Michelle Graham, USDA/ARS-Iowa State University
M A Saghai Maroof, Virginia Tech
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Project Code:
1820-172-0125
Contributing Organization (Checkoff):
United Soybean Board
$671,137
Leveraged Funding (Non-Checkoff):
$ 2.6 million plus the $9.2 million USDA NIFA project that Drs. Maroof/Robertson play roles in.
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Institution Funded:
The Ohio State University
$671,137
Brief Project Summary:

Unique Keywords:
#phytophthora root and stem rot, fusarium root rot, pythium, seedling disease, genetic resistance, #soybean diseases
Information And Results
Project Deliverables

- Release of adapted highly resistant germplasm in the different maturity groups- annually
- Rps gene sequences – manuscripts for Rps2, 3a, and 8, Spring 2017, now Fall 2017
- Mapping of putative novel Rps genes (six), manuscripts and reports to USB in quarterly reports, and to soybean breeders during annual workshop (Feb 2017, now Dec 2017)
- Candidate genes for PI 567301B and PI 567516C with resistance to F. graminearum will be reported – Summer 2017 (presented at Molecular Cellular Biology of Soybean 2016/manuscript published Jan 2017)
- Identify the quantitative trait loci associated with resistance to P. sojae, Pythium spp., and Fusarium graminearum in at least 10 populations.
o Initial mapping of QTL for Pythium spp. (ISU series) Summer 2017, develop additional populations for fine mapping – Student defends Spring 2017.
o Initial mapping of QTL for Pythium spp. (OSU series) Winter/Spring – 2018, develop additional populations for fine mapping
- Candidate gene identification for resistance to these pathogens will be ongoing and reported in USB quarterly reports and given to the group to develop silencing vectors.
o Identify key candidate genes in PI 427106, PI 427105B, V71-370 (chr. 18) for a major QTL for P. sojae. (USB Fellow Stephanie Verhoff).
o Identify key candidate genes in PI 561271 (Chr. 3 and 7) for 2 QTL for P. sojae. (MU)
- Complete transcript analysis of candidate genes in Conrad for P. sojae. Winter 2017.
- Characterize the role of auxin related genes for partial resistance in Conrad – Winter 2017, (USB Fellow – Anna Stasko)
- Characterize the role of auxin-regulated genes for P. sojae partial resistance and its relationship to flooding tolerance in PI 561271 – Winter 2017, MU
- Constructs for silencing candidate genes for both Rps genes and QTL will continue through both years of the study. Initially we are aiming for 20 Rps-related genes and more than 80 QTL related genes. This will also provide specific markers for marker-assisted selection for the key genes.

Final Project Results

Updated December 4, 2018:
See Year 3 for For Final Report (FY19)

See Year 3 for Final Results (FY19)

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.