To investigate the effects of poultry manure on H. glycines abundance, two tons of chicken manure will be applied per acre before corn is planted in 2017. Manure will be applied in 18.2 m strips. In total, four rows will be applied with manure and four rows untreated (two treatments with four replications per treatment). The two treatments will be in alternating rows. Soil samples will be taken before manure is applied, 2-3 weeks after application, and a third sample will be taken near harvest. Soil samples will be taken from each replication as composite samples. Fields will be sampled in a zigzag pattern in order to reduce variability. Nematodes will be extracted and counted from the samples.