Year 3 Milestones and activities (FY16)

Activities 1-4 Discover possible transcription factors that regulate amino acid composition and protein levels. Report results in manuscript format. Additional functional tests by Chip-Seq conducted and networks regulated by two additional transcription factors should be revealed and entered into public databases.

Activity 5. Complete data analysis for the series of three developmental stages of seed cotyledon development using the ribosomal profiling approach of Activity 5. Report the data in manuscript format. These data should tell us the level of translational control for the major seed proteins and the pathways for amino acid synthesis. Enter data into public databases.

Activity 6-7. Complete data generation and analysis. Using this knowledge, transgenic approaches will be initiated to test whether candidate transcription factors that are identified can be used to shift protein and amino acid content. Integrate the knowledge for use by breeders in screening germplasm lines.