2011
Detection of soybean cyst, reniform and root-knot nematodes in soil using multiplex real-time PCR
Contributor/Checkoff:
Category:
Sustainable Production
Keywords:
NematodePest
Lead Principal Investigator:
Ron Sayler, University of Arkansas
Co-Principal Investigators:
Terry Kirkpatrick, University of Arkansas
Project Code:
Contributing Organization (Checkoff):
Institution Funded:
Brief Project Summary:

Currently, the Cooperative Extension Service relies on a time-consuming microscopic process for nematode identification and counting. The researchers propose to develop a PCR-based method to detect soybean cyst, reniform, and root-knot nematode individually and in mixed populations from soil samples. They will incorporate this technology with existing nematode assay methods used by CES to significantly reduce the time and labor required to process the thousands of soil samples received every year and speed reporting of nematode infestations to growers.

Based on these sequences, they will design primers that will be unique and specific for each of the three nematode species, but will...

Unique Keywords:
#nematodes
Information And Results
Project Deliverables

Final Project Results

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.