Updated January 24, 2021:
Final Report
The project discovered the genes (candidates) underlying two high-effect protein and oil QTL that soybean researchers tried to clone for last 28 years. In the FY2020, we applied a combination of quantitative and population genetics, molecular biology, genomics, systems biology and big-data approach to confirm the functions of the two QTL genes in regulating soybean protein and oil. In addition, we generated data supporting that the two QTLs are major genetic components causing the difference in protein and oil in US soybean. They should serve as the most promising gene targets to improve soybean protein content through breeding and biotech. The two QTL genes play important roles in shaping modern soybean as a one of the most profitable crops. We also provide insight into the molecular mechanism on how they regulate the important seed protein and oil traits. We proposed and have been testing a couple of new ideas to use the two genes and their pathways for developing high protein soybean cultivars through traditional gene engineering and new genome editing technologies. A manuscript describing the results and application of the QTL gene (QTL on Chr15) was peer-reviewed and accepted by a prestigious journal in genetics (PLOS Genetics) for publication. The publication allows soybean researchers to access the results broadly and quickly apply the discovers into their research programs for soybean protein improvement.