2020
A highly embryogenic Thorne soybean line
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Contributor/Checkoff:
Ohio Soybean Council
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
John Finer, The Ohio State University
Co-Principal Investigators:
Project Code:
20-R-05
Contributing Organization (Checkoff):
Ohio Soybean Council
$48,000
Institution Funded:
The Ohio State University
$48,000
Brief Project Summary:

Over the past year, a unique and potentially valuable highly embryogenic soybean line of Thorne has been isolated. Embryogenic cultures provide a suitable target tissue to produce transgenic and genome edited soybeans. This line grows 3-5x more rapidly that other lines and shows tremendous potential for improving transformation and genome editing efficiencies. For this research, this line will first be characterized by evaluation of growth under different conditions and then subjected to transformation and genome editing approaches to generate products of use to OSU collaborators. If these cultures show their indicated potential, this research will reduce or eliminate the current bottleneck for producing modified soybean lines.

Key Benefactors:
farmers, agronomists, extension agents, soybean breeders, seed companies

Information And Results
Project Deliverables

Measurable Project Milestones:
The projected start date for this project is 10/1/2019. Milestones targets are quarterly.
1st Quarter, 12/31/2019 – Complete all aspects of Stage 1 – optimize growth conditions of ESE cultures, growth curves, medium compositional analysis, embryo develop studies. Initiate efforts to generate new ESE lines and transformation experiments as these will take a few months to complete.
2nd Quarter, 3/31/2020 – Generate data on development of new ESE lines and transformation efficiency of ‘Thorne’ ESE line.
3rd Quarter, 6/30/2020 – Generation of transgenic lines with genes of interest.
4th Quarter, 9/30/2020 – Generation of genome editing lines with modified genes of interest, Recover transgenic and genome edited plants for analysis.

Final Project Results

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.