2018
Platform for Fast & Cost-efficient CRISPR gene
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Contributor/Checkoff:
Ohio Soybean Council
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Feng Qu, Ohio Agricultural Research and Development Center
Co-Principal Investigators:
Project Code:
18-R-21
Contributing Organization (Checkoff):
Ohio Soybean Council
$25,000
Institution Funded:
The Ohio State University
$25,000
Brief Project Summary:

This project matches a partnership research grant. The ultimate goal is to find cost-effective and sustainable solutions to soybean threats. Recent advancements in the CRISPR-Cas-based genome editing make it possible to tackle these challenges using biotechnological approaches. However, adoption of CRISPR-Cas in soybean is hindered by several roadblocks, including recalcitrance of soybean to transformation, and difficulty to achieve precise gene integration. To address these obstacles, this research pursues the objectives of accelerating soybean gene editing by administering guide RNAs using viruses and adopting Cpf1, a more versatile Cas9 analogue, for gene integration in soybean.

Key Benefactors:
farmers, agronomists, extension agents, soybean breeders, seed companies

Information And Results
Project Deliverables

We expect the successful disruption of the GmPDS1 gene in at least 10% of the leaf cells, and heritable knockout in at least 5% of the seed harvested from the target plants.
We expect to determine the optimal condition that allows us to use Cpf1 to engineer gene integration in the lima bean cotyledon transient system, and to apply this approach for gene integration in Cpf1-transgenic soybean plants.

Final Project Results

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.