2017
Monitor and Manage Soybean Insects FY 17
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Contributor/Checkoff:
Ohio Soybean Council
Category:
Sustainable Production
Keywords:
Biotic stressCrop protectionField management Pest
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Kelley Tilmon, The Ohio State University
Co-Principal Investigators:
Project Code:
17-R-12
Contributing Organization (Checkoff):
Ohio Soybean Council
$60,000
Institution Funded:
The Ohio State University
$60,000
Brief Project Summary:

This project purposes to detect or monitor the distribution of several key soybean pests and beneficials, including stink bugs, kudzu bug, virulent aphid biotypes and pollinator species. This information supports publication of a field guide to stink bugs in Ohio. Research also aims to identify genes that contribute to soybean aphid virulence – the ability to overcome resistant varieties. This leads to the development of molecular markers for biotype diagnostics, as well as improving the ability to predict how durable aphid resistance may be on-farm. Project work also includes mapping soybean resistance to brown marmorated stink bug in PI 097139, a line which shows a low incidence of damage after infestation.

Key Benefactors:
farmers, agronomists, extension agents

Information And Results
Project Deliverables

ProjectMilestonesQ1 (October-December)?Develop trapping maps and outreach material based on the 2016 growing season (current OSC project)?Primers will be designed for aphid virulence genes, and aphids will be exposed through nebulization. We will first test biotype 4 on Rag1/Rag2 pyramid?Grow F2 plant progeny and screen for stink bug resistanceQ2(January-March)?Deliver 2016 trapping and sampling results at various extension venues?We will measure silencing of aphid virulence genes through qRT-PCR?F2:3stink bug screening, DNA extraction in preparation for genotypingQ3(April-June)?Deploy kudzu bug trap sand select sites for stink bug, aphid, and pollinator sampling?All combinations of biotypes and resistant plants will be tested as above.?Plant F2:3plant families for later field screening for stink bug resistanceQ4(July-September)?Conduct insect sampling and trapping and publish relevant real-time information in the C.O.R.N. newsletter?Perform field screening of plants for stink bug resistance; resistance mapping

Final Project Results

Update:
Objective 1: We have identified soybean fields to sample for stink bugs. We have planted aphid resistant soybean lines for screening in various locations in Ohio. We have not detected kudzu bug in any of our first-warning traps. In preliminary sampling through June we have not yet found stink bugs or soybean aphids, though sampling in July and August will continue. For the pollinator survey project covering 8 farmer fields in 7 counties, we continued to sort and identify pollinator species with hopes of finishing this fall. For the study on what time of day pollinators visit soybean, we moved to bee hives to our study site with the help of Dr. Reed Johnson. Objective 2: Stink Bugs of Ohio field guide: 3,000 hard copies of this field guide were printed in February 2018 and distributed to soybean farmers throughout Ohio via extension events and county Extension Educators. Copies for distribution were also supplied to the Ohio Soybean Council. A free online version is also available at the following link. We will further promote this guide during the 2018 growing season. https://aginsects.osu.edu/sites/aginsects/files/imce/Stink%20bugs%20of%20Ohio%20Jan%2018%202018%20Online.pdf Objective 3: We have developed a molecular marker assay with 54 markers and have generated data among soybean aphid biotypes. Objective 4: Plots have been planted and stink bugs have been collected for later infestation

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.