The goal of this project is to develop soybean lines that provide a complete package of feed quality traits for aquaculture production, without the need for additional high-cost diet supplements. Through the use of genetic crossing and emerging synthetic biology strategies, Nebraska soybean germplasm will be developed with economically sustainable levels of EPA and astaxanthin for broad use in aquaculture feed that will be combined with a high vitamin E antioxidant trait that has been previously developed in the PI's lab to provide oxidative stability to extracted soybean oil and increased shelf-life to farmed-fish.