2018
Rhg1, cqSCN Loci and Epigenetic Determinants of Resistance to Soybean Cyst Nematode (1820-172-0117-B)
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Contributor/Checkoff:
United Soybean Board
Category:
Sustainable Production
Keywords:
(none assigned)
Parent Project:
Improved Resistance to Soybean Cyst Nematode via Rhg1 and Other Soybean Loci (Year 1 of 1420-532-5643)
Lead Principal Investigator:
Andrew Bent, University of Wisconsin
Co-Principal Investigators:
Brian Diers, University of Illinois at Urbana-Champaign
Matthew Hudson, University of Illinois at Urbana-Champaign
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Project Code:
1820-172-0117-B
Contributing Organization (Checkoff):
United Soybean Board
$213,436
Leveraged Funding (Non-Checkoff):
Dr. Andrew Bent, University of Wisconsin - Madison. $40,000 from Wisconsin Soybean Marketing Board to generate and test new Rhg1 a-SNAP genes for improvement of soybean SCN resistance, and new transgene-mediated SCN resistance strategies. Dr. Matthew Hudson, University of Illinois at Urbana-Champaign. $90,688 from North Central Soybean Research Program project “An integrated approach to enhance durability of SCN resistance of long-term, strategic SCN management”. $25,000 from I-POC (Illinois Proof of Concept award) to commercialize copy number genotyping assay. Dr. Brian Diers, University of Illinois at Urbana-Champaign. $77,280 from NCSRP project “An integrated approach to enhance durability of SCN resistance of long-term, strategic SCN management”.
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Institution Funded:
University of Wisconsin - Madison
$213,436
Brief Project Summary:

Unique Keywords:
#nematodes, #soybean cyst nematode, scn, genetic resistance
Information And Results
Project Deliverables

• Task 1: By the end of the project year, we will have verified copy number differences identified during the current year, and tested the SCN resistance and obtained at least one field season of agronomic performance data for lines derived from plants with verified difference. Additional screening and selection for plants with copy number variation will be completed.
• Task 2: By the end of the project year we will provide information regarding variable DNA methylation at the Rhg1 locus and its correlation with observed differences in the SCN resistance of different soybean lines that carry similar Rhg1 haplotypes. We will develop and begin testing of assays for Rhg1 DNA methylation and RNA expression that can be used in breeding, with greater emphasis placed on the trait (methylation or RNA expression) that our findings indicate can most successfully be assayed and used to predict relative SCN resistance.
• Task 3: We hope by the end of the proposed project year to have at least preliminarily identified the relevant SCN resistance-conferring gene at one of the PI 468916-derived loci cqSCN006 or cqSCN007. Seed increases of one line with these resistance genes will be in progress to produce seed for commercial sale. One line also will be made available to private industry breeders for use as a parent in breeding programs.
• Task 4: Findings presented at scientific meetings in spring and summer 2017.

Final Project Results

Updated September 11, 2019:

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.