1. Further testing and evaluation of soybeans transformed with desaturases from oyster mushrooms and bread yeast.
2.Transgenic lines with the introduced yeast D9D cloned under the 35S promoter for expression in leaves and resistance to rust and other fungal diseases will be regenerated and grown out in the greenhouse. The seeds will be harvested from these transgenic lines and the seed chips will be analyzed for fatty acid composition and DNA. The lines will be analyzed for segregation and positive ones will be grown out and seed collected and genotyped for rust trials.