2017
Integrating Germplasm Evaluation, Genetic Engineering, Breeding and High-Throughput Phenotyping to Improve Sustainability of Soybean Production
Contributor/Checkoff:
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
William Schapaugh, Kansas State University
Co-Principal Investigators:
William Schapaugh, Kansas State University
Tim C. Todd, Kansas State University
Harold Trick, Kansas State University
+2 More
Project Code:
1730
Contributing Organization (Checkoff):
Institution Funded:
Brief Project Summary:

Objectives of this project include providing high quality germplasm and phenotypic data to develop genome selection tools; identifying new sources of germplasm and genes that improve yield and seed composition; developing superior varieties using new sources of germplasm with improved yield under the extreme environmental conditions; developing high yielding varieties and germplasm lines with desirable levels of protein and oil; developing non-GMO, high oleic soybean varieties and germplasm lines; breeding transgenic events into elite breeding lines; developing sudden death syndrome resistant varieties and germplasm with stacked traits; developing enhanced high-throughput technology to rapidly identify genotypes that have disease and drought resistance, and yield potential.

Key Benefactors:
farmers, breeders, plant pathologists

Information And Results
Project Deliverables

1. In support of an NCSRP project, produce new genotypes and evaluate those genotypes in field evaluations to collect agronomic data used in the development of genomic selection tools. Evaluations will consist of exotic germplasm, elite breeding lines, and advanced lines in the Uniform Soybean Performance Tests.

2.Identify new sources of "good" germplasm. Screen hundreds of different germplasm (exotic germplasm) not contributing to the genetic diversity of US soybean varieties for seed yield, maturity, lodging, shattering, seed composition, and stress response. Genotypic data is also being collected on these accessions in support of Objective 1.

3. Develop superior varieties using exotic germplasm. Each year, develop approximately 40 populations involving exotic parents or lines derived from exotic germplasm with high yielding, elite varieties. Evaluate progeny in both dryland and irrigation field conditions in Kansas and the US and characterize resistance to soybean cyst nematode (SCN) and Soybean Sudden Death Syndrome (SDS). Increase the diversity in SCN populations used in screening trials to represent the natural diversity in SCN populations across Kansas.

4. Develop high yielding soybean varieties with desirable levels of protein and oil. Through a USB project, utilize exotic sources to breed for increased protein and yield. We will also target oil concentrations in our work in Kansas. Each year, develop approximately 15 populations involving exotic parents or lines derived from exotic germplasm with high yielding, elite varieties. Evaluate progeny in both dryland and irrigation field conditions in Kansas and the US and characterize resistance to SCN and SDS.

5. Develop high oleic varieties. Conventional germplasm owned jointly by the USDA and the Missouri Soybean Merchandizing Council is available with high oleic acid. Once an MTA is signed, the high oleic trait will be incorporated into Kansas adapted material through backcrossing and forward crosses.

6. Breed transgenic events into elite breeding lines. In previous funding from KSC, NCSRP, and USB we have produced stable transgenic lines showing enhanced resistance to SCN. Current KSC research is evaluating alternative methods for SDS, SCN and RKN. Together with stable lines already produced we will move these traits to elite breeding lines that have traditional resistances.

7. Develop SDS resistant varieties. Use resistant lines identified from a previous NCSRP SDS breeding project to combine SDS and SCN resistance. Each year, develop approximately 15 populations involving exotic parents or lines derived from exotic germplasm with high yielding, elite varieties. Evaluate progeny in both dryland and irrigation field conditions in Kansas and the US and characterize resistance to SCN and SDS.

8. Develop soybean varieties and germplasm with stacked traits. Each year, soybean lines exhibiting a wide range of desirable traits, including pest resistance, yield stability, and seed quality will be used to develop new populations to produce progeny with combinations of the most desirable traits for Kansas soybean producers, with the ultimate goal of developing varieties with broad pest resistance and yield stability.

9. Develop high throughput technology. Record in-season soybean canopy thermal and spectral profiles with a small unmanned aircraft system (sUAS) at irrigated and dryland research plots. Genotypes possessing extremes in phenotypes (such as disease resistance, wilting and canopy temperature) will be included in the evaluations. Effectiveness of the system will be assessed by correlating thermal and spectral canopy profiles with relative seed yield, relative maturity, and wilting scores. This phenotypic data will be used to support gene mapping.

Final Project Results

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.