2017
Development of w-3 Soybeans
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Contributor/Checkoff:
Kentucky Soybean Promotion Board
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
David Hildebrand, University of Kentucky
Co-Principal Investigators:
Project Code:
Contributing Organization (Checkoff):
Kentucky Soybean Promotion Board
$29,470
Institution Funded:
University of Kentucky
$29,470
Brief Project Summary:

The overall goal of this research project is to increase profitability of soybean production by developing a high ?-3 soybean line for higher value applications. This will be accomplished using a new rapid precision genetic non-GMO approach to soybean improvement/breeding. The specific objectives are: 1.) Test soybean ?-3 desaturases in yeast; 2.) Combine highest activity ?-3 desaturase with a soybean oil body oleosin promoter; 3.) Insert this construct into soybeans & select highest ?-3 somatic embryos and 4.) Grow out highest ?-3 soybeans. This can now be done with a precision genetic approach much faster than traditional breeding but without any non-soybean genes and non-GMO facilitating...

Unique Keywords:
#breeding & genetics
Information And Results
Project Deliverables

With yeast expression of FAD3 genes, we expect to see some variations in desaturase activities among different isozymes from two lines. However, the FAD3C enzyme from the wild soybean is expected to show the highest activity. We expect the engineering of the ‘Jack’ soybean with the FAD3 gene with the highest desaturase activity combined with soybean oleosin or FAD2 gene promoter/terminator will result in the soybeans with much higher 18:3 content. We will further screen their progenies with the selection marker (HPTII from pCAMBIA1301) segregated out so that the final soybean lines will not contain any foreign genes.
The list of deliverables is:
• Soybean FAD3 genes in cloning vectors
• Soybean FAD3 genes in yeast expression vectors
• Yeast colonies with soybean FAD3 genes
• Soybean oleosin promoter sequences in cloning vectors
• Soybean oleosin terminator sequences in cloning vectors
• Soybean FAD2 promoter sequences in cloning vectors
• Soybean FAD2 terminator sequences in cloning vectors
• Vector construct with soybean FAD3 with oleosin promoter/terminator sequences
• Vector construct with soybean FAD3 with FAD2 promoter/terminator sequences
• T1 soybean seeds with high 18:3 contents
• T2 soybean seeds with high 18:3 contents without the HPTII gene

Final Project Results

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.