First quarter (December 2015): Harvest and screen lines segregating for resistance to P. sojae; Pythium and Fusarium. Continue the VIGs experiments for potential R-gene sequence and candidate genes for resistance via functional analysis. Continue to use SNP markers for the Illumina 6KSNP chip for high throughput analysis of additional populations segregating for novel Rpsgenes. Collect soil from studies of management of SCN. Harvest plots and analyze summer data. Complete identification of pathogens from summer/fall surveys. Submit manuscript for Py. irregularepopulation analysis. Begin to evaluate segregating populations for resistance to key Pythium spp.. Train new graduate student on methodologies.
Second quarter (March 2016): Complete screening of all Ohio Breeders material; screening of populations with novel resistance developed/advanced during summer of 2014 as well as isolate DNA for marker analysis. In addition to Phytophthora sojae, screen populations for resistance to Py. irregulare, Py ultimumand compare responses across isolates. Provide breeders with any new data to facilitate decisions required for new soybean populations. Continue with experiments for functional analysis of candidate genes.
Third quarter (June 2016): Initiate summer field studies, begin to increase germplasm with novel resistance genes that require further analysis, evaluate yield effects for specific QTL, raise selected germplasm for studies. Monitor disease development throughout the state, collect isolates for further studies.
Fourth quarter (September 2016) – Collect and summarize summer field data, continue
screening and fine mapping of resistance genes;