Soybean cyst nematode (SCN) is a major yield-limiting factor of soybean. This disease has spread to at least 12 soybean-producing counties in North Dakota. Cultivar resistance is the primary nematode management tactic. SCN is known to be genetically diverse populations and can develop new virulent forms over time due to continuous use of the same sources of resistance. Monitoring virulence changing of SCN populations across the state is important to navigate the use of resistance sources for growers and the new resistance sources that should be introduced to ND. This proposal will determine virulent types of SCN in 50 fields across the state that haven’t been sampled and tested before. The information of HG types will be provided and added to the existing map of SCN distribution and density in ND and available to growers.