2015
Discovery of Novel Nematode Resistance Genes and Development of Productive Soybeans with Multiple-Nematode Resistance (Year 2 of 142-532-5648)
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Contributor/Checkoff:
United Soybean Board
Category:
Sustainable Production
Keywords:
(none assigned)
Parent Project:
Discovery of Novel Nematode Resistance Genes and Development of Productive Soybeans with Multiple-Nematode Resistance (Year 1 of 1420-532-5648)
Lead Principal Investigator:
Zenglu Li, University of Georgia
Co-Principal Investigators:
Silvia Cianzio, Iowa State University
Terry Niblack, The Ohio State University
Bob Robbins, University of Arkansas
Randall Nelson, University of Illinois at Urbana-Champaign
Grover Shannon, University of Missouri
Prakash Arelli, University of Tennessee-Institute of Agriculture
+5 More
Project Code:
1520-532-5648
Contributing Organization (Checkoff):
United Soybean Board
(n/a)
Institution Funded:
University of Georgia
(n/a)
Brief Project Summary:

Unique Keywords:
#breeding & genetics
Information And Results
Project Deliverables

Objective 1. Application of biotechnology to control of the soybean cyst nematode: Genetic diversity and soybean genetic resources (P. Arelli, S. Cianzio, and Z. Li)

1) Identify new sources of SCN resistance
2) Release conventional cultivars and germplasm lines with SCN resistant in MG I, II, and III (Cianzio, ISU)
3) Release soybean germplasm lines with SCN and RKN resistance in maturity groups VI. VII and VIII (Arelli, USDA and Zenglu Li, UGA)
4) Improved germplasm lines with SCN resistance from Peking source
5) Improved DNA marker assay for selection of SCN and RKN resistance

Objective 2: Genetic evaluation and development of germplasm resistant to multi-nematodes in group III, IV, and V soybeans (H. Nguyen, G. Shannon, T. Vuong, Z. Li and R. Robbins)

1) The development of several mapping populations derived from new sources of resistance to SCN, RKN, and RN.
2) Completion of QTL analyses of RKN and RN resistance in the Magellan x PI 567516C population.
3) Completion of QTL analyses of SCN, RKN and RN resistance in the new population derived from the Magellan x PI 567305 population.
4) Completion of the evaluation of over 500 PIs from early MG for SCN resistance.
5) Completion of association mapping study using the USB-supported SNP marker development (~50,000 SNPs) to detect novel genomic regions conveying resistance to SCN.
6) Narrowing down the genomic regions of novel QTL by fine-mapping.
7) The development of molecular markers associated with the genes/QTL identified and test high-throughput SNP genotyping platform using the Fluidigm EP1 system.
8) Robust SNP marker assays for selection of root-knot Ma and Mj resistance

Objective 3: Screen lines derived from crosses between soybean and G. tomentella and identify lines that have SCN resistance transferred from the G. tomentella parent. (R. Nelson, R. Singh, and T. Niblack)

1) Soybean lines with SCN resistance derived from introgressed G. tomentella.
2) Evidence in the form of DNA markers and response to HG types that indicates the SCN resistance from G. tomentella is different from that currently available from soybean or wild soybean.




Final Project Results

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.