We will expect to produce a working SCN virulence assay validated on various unrelated SCN populations. While our work, over the next year may not develop a DNA-based virulence test for all known Hg Types, we hope to have assays for some of the most common virulence types. We will also produce a list of soybean resistance gene combinations that show differing selection of SCN virulence loci. Finally we will produce a list of soybean proteins that bind to our verified SCN virulence protein.