The primary deliverables for this project are:
a) Knowledge regarding the Rhg1 locus encoding soybean resistance to soybean cyst nematode, such as information on available Rhg1 locus variation in soybean germplasm, the impact of copy number of SCN resistance, the nature of chemical changes in soybean when Rhg1 is expressed, and the relative contributions of different Rhg1 genes to SCN resistance in transgenic soybean tissues.
b) Scientific publications regarding Rhg1.
c) Patent filings or addenda to filed patents regarding Rhg1.
d) Soybean germplasm carrying potentially useful variant forms of the Rhgl locus.
e) Marker technologies to assess Rhg1 copy number or other Rhg1 genotype data.
f) Refinement of the genetic map position and/or gene identity information for at least one of the two identified SCN resistance QTLs from PI 468916.

Updated November 8, 2017:
• Attempts to identify a soybean line containing Rhg1 resistance from both Peking and PI88788 types is ongoing, but has not yet been identified.
• Analysis of F2 plants derived from crossing parents with different rhg1 copy number and type as well as presence and absence of Rhg4, confirms and extends our understanding of these genes. It appears that for Hg2.5.7 populations, Rhg1 derived from Peking provides more resistance than does Rhg1 derived from Fayette, Rhg4 does not impact resistance in plants with Fayette + Williams Rhg1 but increased copi
• Work to identify proteins in soybean that physically interact with Glyma18g02610 using metabolite profiling is ongoing. Subcellular localization of Glyma18g02610 suggests it is primarily in the cytosol, but may also be associated with the plasma membrane.

The knowledge that the number of copies of the gene Rhg1 may have bearing on the magnitude of SCN resistance within a variety will make a difference in the future of how varieties are bred for improved SCN resistance.