1. Finish the development of over 20 RIL mapping populations derived from new sources of resistance to SCN, RKN, and RN. Continue to produce new crosses based upon unique haplotypes of these PIs.
2. Completed genotyping two new F7 RIL populations using the whole genome re-sequencing technology, leading to discovery and mapping of new sources of SCN, RKN, and RN genes/QTL.
3. Use haplotype data (Soybean SNPs) to study previously reported genomic regions on Chr. 10 (LG-O), Chr. 18 (LG-G), and Chr. 6 (LG-C2).
4. Conduct association mapping study using the USB-supported SNP marker development (~50,000 SNPs) to detect novel genomic regions conveying resistance to SCN.
5. Initiate the development of molecular markers associated with the genes/QTL identified and test high-throughput SNP genotyping platform using the Fluidigm EP1 system.