The proposed research will produce molecular research tools and a viable strategy for generating clean (selectable marker-free, recombinase-free) transgenic soybean plants. Novel soybean transformation vectors will have been constructed and successfully used to generate independent founder lines which will form the basis for future soybean biotechnology research. If successful, these resources will enable the production of single copy transgenic soybean, free of selectable markers and any other unwanted transformation sequences, in an efficient single-step genetic engineering process.