2013
Tagging Rag1 Virulence in the Soybean Aphid with DNA Markers (#0243) and Addendum Project (#1243): Genetics of Resistance …(Year 2 of 2243)
Contributor/Checkoff:
Category:
Sustainable Production
Keywords:
(none assigned)
Lead Principal Investigator:
Curtis Hill, University of Illinois-Carbondale
Co-Principal Investigators:
Project Code:
1320-532-5625
Contributing Organization (Checkoff):
Institution Funded:
Brief Project Summary:

Unique Keywords:
#insects and pests, #soybean gene expression, #soybean insect - genetic resistance
Information And Results
Project Deliverables

Soybean scientists such as breeders and entomologists in academic institutions and soybean seed companies will directly utilize the marker technology and information on marker association with Rag1 virulence to screen soybean aphid populations for the potential to colonize plants with the Rag1 soybean aphid resistance gene. Seed companies and other soybean aphid scientists will use the marker technology to determine where to market cultivars with Rag1 and will help extension entomologists determine the risks to growers of planting cultivars with or without Rag1. Information on virulence potential will also help soybean producers select appropriate resistant cultivars or to determine whether to employ alternative soybean aphid management practices instead. This information will directly influence the soybean producer’s decision whether to select a cultivar with Rag1 or not. Use of this strategy to identify Rag1 virulence potential in aphid samples will increase the effectiveness and durability, or length of useful service, of Rag1 in production. Effective use of Rag1, along with other suitable management practices, can significantly stabilize soybean production, especially when there are serious aphid outbreaks.

Information on the novel aphid resistance genetics in PI 587677 and PI 594592 will be released to public and private soybean breeders, under license. This information will facilitate the production of new soybean cultivars with a wider spectrum of resistance to the soybean aphid. The addition of new soybean aphid resistance genetics, derived from PI 587677 and PI 594592, will contribute to the overall integrated management of the soybean aphid.

Final Project Results

• A 2,573 SNP array for soybean aphid genes was developed that will allow mapping of soybean aphid biotype distribution
• Reciprocal crosses were made between aphid biotype 1 and 2 to determine inheritance of aphid virulence and a maternal influence was determined
• A single dominant resistance gene for aphid biotype 3 was identified in PI 587677 and two complementary genes were found in PI 594592.
No achieving the objectives
• Additional crosses were required among PIs with Rag1 and Rag2 resistance genes to determine allelism.
• More research is necessary to determine the source of virulence to Rag1 in soybean aphid due to the finding of strong maternal influence.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.