2013
Screening for genetic resistance against soybean viruses
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Lead Principal Investigator:
John Hill, Iowa State University
Co-Principal Investigators:
Steve Whitham, Iowa State University
Reza Hajimorad, University of Tennessee-Institute of Agriculture
Craig Grau, University of Wisconsin
+2 More
Project Code:
Contributing Organization (Checkoff):
Institution Funded:
Brief Project Summary:

Previous research has found that the control of the disease caused by bean pod mottle virus (BPMV) could not be achieved through management of the virus vector, the bean leaf beetle. Therefore, the most practical and reliable method for disease control is disease resistance. The goal of this project is to identify resistant BPMV soybean lines that can be used by soybean breeders.

Hill and Grau developed a novel seed-based assay to measure the amount of virus in a seed to identify BPMV field tolerance. That is coupled with a method for BPMV resistance screening which involves an engineered BPMV isolate that contains a gene for herbicide resistance (subsequently referred to as the BPMV-BAR...

Unique Keywords:
#alfalfa mosaic virus (amv), #bean pod mottle virus (bpmv), #breeding & genetics, #soybean diseases, #soybean stress-genetic resistance
Information And Results
Project Deliverables

Final Project Results

Similar to the challenge associated with BPMV, there are few known sources of natural resistance against AMV in soybean (Crop Sci. 48:933). We have unequivocally established the seed transmissibility of AMV in soybean. This is important since AMV infected seed could serve as an inoculum source for spread of soybean-adapted variants to a new area. The antibody-based ELISA method developed in the laboratories of Drs. Hill and Grau for finding sources of resistance against BPMV and SMV by screening seeds collected from field-grown soybean plants is likely applicable to AMV.

However, the major limitation in application of this approach to AMV was lack of a suitable immunoassay. Suitable antibodies against AMV were produced that can easily detect a variety of virus isolates in infected plants. The results pertinent to these antibodies are published. Methods for extraction of seeds for the ELISA were determined in order to maximize the sensitivity of the assay. However, screening of soybean seeds for the presence of virus is awaiting availability of adequate seeds collected from AMV infected fields. These seeds are going to be produced in the current growing season.

For field testing of plants for resistance screening, it was determined that for North Central producers samples should consist of pooled trifoliolate leaf samples taken 30 - 60 days after infection. Symptomless infection can occur which means assessment of resistance and/or seed transmission based on field symptoms is problematic.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.