2015
Generation and use of novel soybean germ plasm with enhanced SDS resistance
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Contributor/Checkoff:
Iowa Soybean Association
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
Generation and use of novel soybean germplasm with enhanced SDS resistance
Lead Principal Investigator:
Madan Bhattacharyya, Iowa State University
Co-Principal Investigators:
Silvia Cianzio, Iowa State University
Project Code:
Contributing Organization (Checkoff):
Iowa Soybean Association
(n/a)
Institution Funded:
Iowa State University
(n/a)
Brief Project Summary:

The goal of this project is to create SDS-resistant soybean cultivars for enhancing profitability of Iowa soybean growers. The approach is to isolate a nonhost resistance gene from Arabidopsis thaliana and transfer to soybean. The work maps and isolates Arabidopsis genes that confer broad-spectrum resistance to both the SDS pathogen, Fusarium virguliforme and the root rot pathogen, Pytophthora soja. It then generates transgenic soybean lines containing those genes and evaluates them for expression of possible SDS and Phytophthora resistance. Then, the plan is to make crosses of selected transgenic lines with SDS and/or Phytophthora resistance to elite soybean lines.

Key Benefactors:
farmers, agronomists, Extension agents, soybean breeders, seed companies

Information And Results
Project Deliverables

1. Genetically map Pss19 that confer immunity of Arabidopsis against both P. sojae and F. virguliforme. (Note: this deliverable is in addition to what were listed in the original proposal).
Timeline: Complete this task by March, 2015.
2. Transgenic soybean plants carrying Pss21 and/or Pss25 will be created and evaluated for possible enhanced SDS resistance.
Timeline: Evaluate progenies of transgenic plants starting March, 2015.
3. First cross of the selected homozygous transgenic soybean lines with elite soybean lines is expected to be initiated.
Timeline: Progenies of the transgenic plants are expected to be available in April, 2015. The progenies of the transgenic plants showing SDS resistance will be crossed to an elite line in August-September, 2015.

Final Project Results

1. Genetically map Pss19 that confer immunity of Arabidopsis against both P. sojae and F. virguliforme. (Note: this deliverable is in addition to what were listed in the original proposal).
Progress: We have mapped the Pss19 gene to the Arabidopsis Chromosome 4 between markers SBP_6.51 and SBP_2.91. The gene co-segregates with molecular markers NGA8 and SBP_5.6. We have submitted a pooled DNA sample from eight susceptible families of a cross for sequencing at the DNA facility, Iowa State University (ISU). Currently, we are analyzing the genome sequence data to identity the candidate Pss19 gene.

2. Transgenic soybean plants carrying Pss21 and/or Pss25 will be created and evaluated for possible enhanced SDS resistance.
Progress: Soybean transformation has been conducted at the Plant Transformation Facility, ISU. Three fusion genes for each of Pss21 and Pss25 genes were generated by fusing each gene to CaMV 35S promoter and promoters (Prom1 and Prom2) of two soybean genes that are expressed in roots and induced following infection. We now have transgenic soybean plants in greenhouse for four of the six fusion genes. Currently, we are harvesting seeds (Ro) of the transgenic lines for four constructs carrying Pss21 and Pss25. Remaining two constructs (P1-Pss21 and 35S-Pss25) are being used to develop transgenic lines.

3. First cross of the selected homozygous transgenic soybean lines with elite soybean lines is expected to be initiated.
Progress: Since transformation and characterization of the transgenic plants were not yet completed, we could not make any crosses this summer as proposed earlier.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.