2014
Utilization of VIGS to identify resistance to agents of stress in soybean
Contributor/Checkoff:
Category:
Sustainable Production
Keywords:
Crop protectionDiseaseField management
Lead Principal Investigator:
John Hill, Iowa State University
Co-Principal Investigators:
Thomas Baum, Iowa State University
Steve Whitham, Iowa State University
+1 More
Project Code:
Contributing Organization (Checkoff):
Institution Funded:
Brief Project Summary:

This research team continues to develop a high throughput gene silencing technology (VIGS - virus induced gene silencing) system that can be used to develop durable resistance against a multitude of soybean disease-causing agents. The project involves placing the VIGS system with multiple investigators to help understand functional genomics. The ultimate goal of this project is to identify genes that can be used to develop new resistance traits for different pathogens and to identify new genetic markers for disease resistance that can be used by soybean breeders.

Unique Keywords:
#genetic resistance to diseases, #soybean diseases, #soybean gene mapping, #soybean gene markers, #virus-induced gene silencing
Information And Results
Project Deliverables

Final Project Results

For SCN, two approaches are being continued to characterize the plant-nematode interaction. These are silencing genes in soybean roots that potentially play an important role in the plant-nematode interaction and to determine if BPMV replication can be documented in the syncytium with the ultimate goal of targeting silencing to the feeding site.

In tests with SDS, tests of genes belonging to the ethylene mediated defense response pathway and the jasmonic acid biosynthesis pathway have been repeated. Additional constructs expected to target the same pathways were added to an expanded test.

Approximately 190 plants were screened using the BPMV VIGS system in screens for Asian soybean rust. Candidate gene screening of Rpp3 plants suggested potential for loss-of-resistance phenotype. Three BACs, of which two have been sequenced, have been identified that partially span the Rpp1 resistance locus.

Unexpectedly, tests with soybean mosaic virus revealed that incorporation of the marker gene GUS could alter the avirulence/virulence profile of the virus. Since this gene was being used as a marker gene in studies of the impact of BPMV constructs on soybean mosaic virus pathogenicity, we have elected to use wild type soybean mosaic virus in assays. This has allowed identification of 14 BPMV constructs that may be of interest, although further confirmation of this data is necessary.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.