1. First batch of 50 germplasm lines were planted in the green house. Leaf and root tissue samples were collected from these lines.
2. 50 seed of each line was grown in the green house and the plants were thinned to 24 plants and leaf tissue was collected from each of the 24 plants and pooled.
3. Good quality DNA has been extracted from these samples.
4. DNA samples were shipped to Perry Cregan lab for genotyping using 50K SNP array to determine PI line purity and homogeneity testing.
5. The reference genome line, “Lee” has been planted in the greenhouse. Samples will be collected for the DNA processing.
6. More germplasm lines are currently growing in the greenhouse for tissue collection and DNA extraction for sequencing work.
7. Bioinformatics group is analyzing the sequence data available from other research projects. These analyses help standardize the parameters on data quality, analysis pipeline, and result output.

This project was initiated preceding the incorporation and standardization of KPIs. The progress of objectives are as follows:
Achieving objectives:
• The data analysis for 50 lines at 40X coverage and 300 lines at 15X coverage for Phase I is continuing.
• Industry partners have been sent 15X data from 300 additional lines for internal analysis.
• The first step of sequencing cultivar Lee using reference guided assembly has been completed. Analysis of the sequencing and quality threshold determinations are ongoing. Quotes for the second step, de-novo assembly, have been received.
• Tissue for 396 out of the next 408 lines has been collected. DNA extraction for 200 lines is completed. These have been sent to Washington University for heterogeneity testing.
• A group project meeting was held on January 10, 2015 at PAG in San Diego.

Not achieving objectives:
• None at this time.