We advanced two mapping populations by two generations in Costa Rica over the winter and planted F5 plant rows at Urbana this summer. This included 326 plant rows from Dwight x LG11-3187 and 350 plant rows from Dwight x LG11-12313. Using DNA markers we confirmed that all F1 plants from which these lines were derived were from cross-pollinations. The seeds were delayed coming back from Costa Rica and were not planted until July 2. Excellent conditions for emergence, a good growing season and a warm fall allowed for a reasonable harvest. DNA was sampled from each row and currently each line is being sequenced. The reduced yield because of the very late plating will limit the amount of testing that we will be able to do in 2016.

We evaluated 800 recombinant inbred lines derived from crossing PI 483916 and PI 479752 with Williams 82 in 2 replications at two locations in 2015. We collected data on time of flowering, stem diameter, leaflet size, leaflet shape, pubescence orientation, lodging, height, plant type, time of maturity, and shattering. Seed samples from all plots were harvested and data on seed traits will be collected this winter. Genotyping by sequencing was completed on the approximately 100 lines that had not previously been sequenced.

The 416 experimental lines derived from wild soybean that we selected based on observations on over 1,700 plant rows in 2014 were evaluated in 2015 at two locations. We collected data on maturity, lodging, height and yield.

We have seed from 326 recombinant inbred lines from Dwight x LG11-3187 and 350 recombinant inbred lines from Dwight x LG11-12313. In our previous yield testing LG11-3187 (F6 Dwight (4) x PI 441001) yielded 7.6 bu/a acre more than Dwight and was 6 days later in maturity than Dwight. LG11-12313 (F3 Dwight (5) x PI 441001) yielded 3.6 bu/a more than Dwight and was 1 day later in maturity. These yield mapping populations are ready for yield testing in 2016 and the results from that research will indicate the genetic introgressions from G. tomentella to soybean that has caused these very large yield increases.

We completed the third year of field data collection for the 800 recombinant inbred lines derived from crossing PI 483916 and PI 479752 with Williams 82. We have also completed the genotyping by sequencing for all 880 lines. Seed samples were harvested from the 2015 plots for classification and analysis.

The 416 experimental lines derived from wild soybean that we selected based on observations on over 1,700 plant rows in 2014 were evaluated in 2015 at two locations. We collected data on maturity, lodging, height and yield.