2015
Accelerating soybean yield improvement by utilizing yield genes from soybean wild relatives
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Randall Nelson, University of Illinois at Urbana-Champaign
Co-Principal Investigators:
Jianxin Ma, Purdue University
Patrick Brown, University of Illinois at Urbana-Champaign
Brian Diers, University of Illinois at Urbana-Champaign
Matthew Hudson, University of Illinois at Urbana-Champaign
Ram Singh, University of Illinois-Carbondale
George Graef, University of Nebraska
+5 More
Project Code:
Contributing Organization (Checkoff):
Institution Funded:
Brief Project Summary:

To achieve yield gains in soybean, increasing the diversity available to soybean breeders is a strategy that has much promise. Preliminary data is available that indicate that very useful yield genes exist in both annual (Glycine soja) and perennial (Glycine tomentella) wild relatives of soybean (Glycine max) that are not being used in commercial soybean breeding.

The perennial Glycine species are so different from soybean that they are not considered to be "wild soybeans." They are native to Australia and survive in some very harsh environments. The genetic differences between perennials and annuals are so large that the soybean cannot be crossed with the perennial Glycine using...

Unique Keywords:
#breeding & genetics, #glycine soja, #glycine tomentella, #soybean diversity, #soybean genetic mapping, #yield
Information And Results
Project Deliverables

We advanced two mapping populations by two generations in Costa Rica over the winter and planted F5 plant rows at Urbana this summer. This included 326 plant rows from Dwight x LG11-3187 and 350 plant rows from Dwight x LG11-12313. Using DNA markers we confirmed that all F1 plants from which these lines were derived were from cross-pollinations. The seeds were delayed coming back from Costa Rica and were not planted until July 2. Excellent conditions for emergence, a good growing season and a warm fall allowed for a reasonable harvest. DNA was sampled from each row and currently each line is being sequenced. The reduced yield because of the very late plating will limit the amount of testing that we will be able to do in 2016.

We evaluated 800 recombinant inbred lines derived from crossing PI 483916 and PI 479752 with Williams 82 in 2 replications at two locations in 2015. We collected data on time of flowering, stem diameter, leaflet size, leaflet shape, pubescence orientation, lodging, height, plant type, time of maturity, and shattering. Seed samples from all plots were harvested and data on seed traits will be collected this winter. Genotyping by sequencing was completed on the approximately 100 lines that had not previously been sequenced.

The 416 experimental lines derived from wild soybean that we selected based on observations on over 1,700 plant rows in 2014 were evaluated in 2015 at two locations. We collected data on maturity, lodging, height and yield.

Final Project Results

We have seed from 326 recombinant inbred lines from Dwight x LG11-3187 and 350 recombinant inbred lines from Dwight x LG11-12313. In our previous yield testing LG11-3187 (F6 Dwight (4) x PI 441001) yielded 7.6 bu/a acre more than Dwight and was 6 days later in maturity than Dwight. LG11-12313 (F3 Dwight (5) x PI 441001) yielded 3.6 bu/a more than Dwight and was 1 day later in maturity. These yield mapping populations are ready for yield testing in 2016 and the results from that research will indicate the genetic introgressions from G. tomentella to soybean that has caused these very large yield increases.

We completed the third year of field data collection for the 800 recombinant inbred lines derived from crossing PI 483916 and PI 479752 with Williams 82. We have also completed the genotyping by sequencing for all 880 lines. Seed samples were harvested from the 2015 plots for classification and analysis.

The 416 experimental lines derived from wild soybean that we selected based on observations on over 1,700 plant rows in 2014 were evaluated in 2015 at two locations. We collected data on maturity, lodging, height and yield.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.