2010
Construction of a DNA-based virus induced gene silencing system for functional genomics of soybean seed development
Category:
Sustainable Production
Keywords:
GeneticsGenomics
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Leslie Domier, USDA/ARS-University of Illinois
Co-Principal Investigators:
Said A Ghabrial, University of Kentucky
Project Code:
Contributing Organization (Checkoff):
Institution Funded:
Brief Project Summary:

As one of the most important seed crops in the world, an understanding of the regulatory networks that shape the biochemical properties of soybean seed are becoming increasingly important. Virus induced gene silencing (VIGS) is a powerful tool for functional genomics that permits inactivation of individual genes or closely related gene families. Inactivation of genes through VIGS is most effective in cells where recombinant viruses replicate. Tobacco streak virus (TSV), a virus that commonly infects soybean, readily invades meristematic tissues and developing soybean embryos, which results in high rates of seed transmission (often >50%). Consequently, VIGS vectors based on TSV would permit...

Unique Keywords:
#breeding & genetics, #soybean mosaic virus (smv), #tobacco streak virus (tsv), #virus-inducted gene silencing
Information And Results
Project Deliverables

Final Project Results

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.