2013
The role of GH3 genes in plant resistance against charcoal rot disease
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Contributor/Checkoff:
Kansas Soybean Commission
Category:
Sustainable Production
Keywords:
Crop protectionDiseaseField management
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Bin Shuai, Wichita State University
Co-Principal Investigators:
Project Code:
Contributing Organization (Checkoff):
Kansas Soybean Commission
(n/a)
Institution Funded:
Wichita State University
(n/a)
Brief Project Summary:

Charcoal rot is a plant disease caused by soil-borne fungus Macrophomina phaseolina. In Kansas, the crop most severely affected by charcoal rot is the soybean, especially in the southeast and east central regions of the state. In this research, we will confirm the full length sequences of GH3 cDNAs by 5'-and 3'-RACE and identify GH3 genes that respond to auxin and M. phaseolina infection.

Unique Keywords:
#charcoal rot, #macrophomina phaseolina, #soybean diseases, #soybean genomics
Information And Results
Project Deliverables

Final Project Results

The first aim is still being optimized. The second aim has successfully been completed. It applied real-time quantitative PCR to identity GH3 genes that respond to auxin treatment and M. phaseolina infection. Based on the results, we identify GH3.2, GH3.3 , and GH3.5 as auxin-inducible, all of which showed more than 2-fold increase in expression when plants were treated with IAA. M. phaseolina infection resulted in rapid increases in expression of GH3.2, GH3.3, GH3.4 and GH3.7. However, the induction seems to be transient for GH3.4 and GH3.7, which showed decreased expression at 48 hpi and 36 hpi respectively. To further understand the role of these GH3 genes in disease resistance, we have applied funding to support the second year of the project that uses a genetics approach to examine the effect of GH3 gene mutations on plant disease responses to M. phaseolina.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.