Soybean cyst nematode (SCN) is a major threat to soybean crop, causing significant yield losses. In North Dakota (ND), since its first detection in 2003 in Richland County, SCN has been spreading rapidly. SCN can become ND soybean growers’ biggest disease problem if it is not detected early and managed proactively. Early and accurate detection of SCN is crucial for its effective and timely management. Traditional methods of detection include visual inspection, soil sampling, microscopic identification of morphological features, and manual counting of cysts and eggs, which is very labor intensive, time consuming, and requires specialized training. The NDSU nematology lab and other nematode diagnostic labs have invested tremendous time and money for SCN cysts and eggs identification and counting from numerous samples collected from soybean fields and from greenhouse and field experiments using the traditional methods. In recent years, research on utilization of artificial intelligence (AI) in different sectors has gained a momentum with rapid advancement and widespread adoption across various sectors including agriculture science. However, AI detection and quantification of SCN haven’t been evaluated in North Dakota.