Soybean meal, with its complete amino acid profile, is an excellent protein source for livestock. However, soybean meal cannot be directly used for animal feed mixtures due to the presence of various anti-nutritional compounds which reduce animal weight gain. There are two major proteinaceous anti-nutritional factors in soybean seeds: 1) Kunitz-trypsin inhibitors (KTi) which inactivate the animal digestive enzyme trypsin; and 2) Bowman-Birk inhibitors (BBi) which inactivates both trypsin and chymotrypsin animal digestive enzymes. Earlier, we have developed soybean germplasm that has the lowest levels of trypsin inhibitor reported for conventional soybeans. However, it still retains significant levels of trypsin inhibitor activity, due to the presence of BBi proteins. Therefore, it is essential to remove both KTi and BBi for the purpose of developing soybean lines that would require extremely low or no processing. With USB funding, we have for the first-time developed soybean lines with almost undetectable amounts of BBi in the seeds by gene editing technology. We have also steadily progressed towards generating KTi knock-out lines by CRISPR/CAS9 technology. However, we are still awaiting the delivery of KTI knockout transgenic soybean lines from the University of Missouri Plant Transformation Core facility. Additional research is needed to stack these two traits (absence of KTi and BBi) into a single soybean line.