Throughout this project we advanced transgenic plants for two generations to T5 seeds. Along the way, we have characterized the growth and the seed amino acid and total crude protein concentrations in two independent events of each of our four constructs. Our analyses revealed impressive increases in crude protein content for all four constructs and all independent events, with increases ranging from 11 to 22% relative to the control. The concentrations of individual amino acids also were altered with particularly large relative increases observed for Arginine and Hydroxyproline whereas relative Cysteine and Methionine concentrations were reduced. These results are very intriguing in that all constructs mediated changes in the amino acid composition and increased seed crude protein. However, the way the constructs caused the observed differences needs to be further investigated as the modification in the amino acid concentrations were unexpected. To end, we have planted another set of plants to: i) generate sufficient seeds for more detailed studies of processes that modulate the seed amino acid concentration, and ii) further scale up the amount of seed available for field studies which are needed to establish whether the transgenic events result in the same increases in crude protein and changes in amino acid concentrations under field conditions as the due under controlled conditions.