Trypsin inhibitor (TI) is a major anti-nutritional protein in soybean meal. Since soybean meal with high TI has a negative effect on the animal performance, it has become increasingly important for livestock feed producers to reliably measure the TI activity in their feed. However, routine measurement of trypsin inhibitor activity is not trivial due to cumbersome steps involved in measuring TI activity using the current methods. Currently, trypsin inhibitor activity is measured utilizing the “standard method” that has been approved by American Oil Chemists Society (Method Ba 12-75) and American Association of Cereal Chemists International (Method 22-40.01). With USB funding, we have modified and improved this “standard method” resulting in the elimination of several time-consuming steps and drastically reducing the assay volume. We obtained greater than 500 soybean meal samples from industry partners and measured the TI activity in these samples. Trypsin inhibitor analysis of these samples at different times and by different lab personnel produced similar results thereby validating the reproducibility of our assay. The results of our trypsin inhibitor measurement have been communicated to all the industry partners who provided the SBM samples. Importantly, our results indicate that most of these US SBM samples contain relatively low levels of trypsin inhibitor activity. We have received positive feedback from several of our industry partners and we trust that our simple, fast, and reliable trypsin inhibitor activity assay will be widely adopted.