Progress has been made in assessing the potential effects of rising temperature on soybean seed yield and seed quality. We continue to test germplasm for heat stress response in our Temperature Gradient Greenhouse. In 2023, we screened advanced breeding lines developed from crosses between domesticated soybeans and soybean ancestors for response to elevated temperature to identify potential sources of stress tolerant traits contributed by ancestral germplasm. This complements previous work on screening commercial cultivars and elite drought tolerant lines developed by the USB Drought Team for heat stress response. Certain genotypes perform well under elevated temperatures in the range of +2 to +4 Celsius while others exhibit significant yield losses. This represents sufficient genetic variation in response to allow identification and incorporation of heat tolerance traits for soybean improvement.