Saponins are a family of bitter tasting secondary metabolites that are also a causative agent of digestive distress in aquaculture-raised fish such as salmon. Previously, we have generated BE and non-BE low saponin traits through gene-editing and chemical mutagenesis, respectively. Here, we established a highly sensitive method to quantify saponin levels, which allowed us to confirm our low saponin traits. We started introgressing our low-saponin trait into high-protein, low-oligosaccharide germplasm to increase the value of the low-saponin trait. Currently, our conversions are at backcross 2. In addition, we have conducted sensory testing of the low-saponin trait in the form of a heated 10% soy slurry to evaluate our low-saponin soybean in food applications. The low-saponin trait had no significant effect on bitterness which did not meet our expectations. Next, we will complete introgression of the low-saponin trait, simultaneously increase seed for efficacy and equivalency studies, and conduct fish feeding trials for aquaculture application.