Our previous research demonstrated there is an opportunity to enhance soybean productivity and seed composition with management practices that increase late-season N availability. We conducted follow up multi-state trials in 2022 and 2023 to quantify if there is a minimum N fertilizer rate that is efficient in increasing soybean productivity and seed protein concentration. We employed aerial imaging technology to monitor differences in the plant growth and N status. We collected frequent destructive samplings to monitor different tissue growth and composition. We also collated and analyzed soybean yield and seed composition data from eight long-term trials across the US evaluating different rotation, tillage, and fertilizer practices. We will now finalize tissue and data analysis, and utilize data to improve process-based crop model simulations of soybean yield, crop growth and nitrogen, and seed composition. Outcomes from this research will deliver environmentally sustainable management recommendations and novel decision tools that contribute to increasing soybean seed yield and quality.