Traditional oil extraction methods such as solvent extraction and mechanical pressing have drawbacks, notably in resource wastage and denaturing proteins that are by-products of the extraction process. Our project investigates aqueous extraction as an alternative method that could circumvent these issues. This method is gentler and helps preserve the quality of the oil and the proteins, which have been applied in fruit oil extraction. The major challenge with this method when applied to soybeans is the lower yield of oil. This is due to the oil being present in soybeans is mainly in conjugated forms, intermixed with other substances. To tackle this, our lab is working on using special enzymes to break down these complex forms and release more oil. These enzymes include protease, carbohydrase, and phospholipase. We are also employing a technique called ball milling, which helps in making these enzymes even more effective. Our goal is to develop an aqueous-enzymatic method for soybean oil extraction, offering alternative choices for industrial oil extraction. The current project focuses on finding the best combination of enzymes and the right conditions to maximize the oil yield. This approach holds the potential to render soybean oil extraction more sustainable and reduce the generation of low-profit by-products.