Hypothesis: Winter cover crops, will act as green mulch during the growing season, alleviating salinity problems, obtaining significantly soybean higher yields
Objectives:
• To determine the cover crop seeding rate to improve soybean yield in saline conditions
• To determine termination cover crop date, to get the highest soybean yield in saline soil conditions.
Methodology to attain objectives
Experimental design: The experiment will be conducted in Carrington for two years for soybean-cover crops. The trial will be established under dryland. The experimental design will be a randomized complete block design (RCBD) with a factorial arrangement with four replicates (Table 1). The first factor will be five cover crop treatments: soybean (check), soybean and winter rye, winter barley, winter camelina, and a cover crop mix. The second factor will include cover crops, with 66% and 33% of the recommended rate, respectively. The third factor will be the cover crop termination date, the first will be cover crop termination with glyphosate at R2 soybean stage, and the second without termination: The experimental unit will be 10 ft x 25ft.
Table 1. Treatment structure
Salinity mapping: A salinity map will be made in the trail area, using EM38, and apparent electrical conductivity (ECa) will be recorded and mapped in ArcGIS, with the idea of identifying the salinity gradient in the soil, facilitating the field design and analysis.
Soil sampling: Composite samples will be taken at 0-6 and 6-24 inch depth in early spring, with the the top soil to be analyzed for NO3-N, pH, P, K, Sulfate-S, Zinc, pH, EC, and organic matter, and samples taken at 6-24 inches will be tested for NO3-N and Sulfate-S. The results will be used to determine the P, K, and S fertilizer application. In-season soil sampling for electrical conductivity and pH will be done at the R2 soybean stage at the 0-6 inch depth.
Plant Sampling: Mid-season biomass samples will be taken from a 1 m section of an internal row at the R2 soybean growth stage. These samples will be dried and weighed to determine biomass production. At the same time, an above-ground biomass sample of the cover crop will also be taken. Shortly before soybean harvest, cover crop biomass samples will be taken in all plots where glyphosate was not sprayed. At soybean harvest, grain yield, test weight, grain protein, and grain oil data will be collected.
Weather and soil data: Daily temperature (min and max), relative humidity, and rainfall will be obtained from NDAWN stations. Soil moisture and temperature will be obtained from 1 rep of the experiment with a Decagon 5TM soil moisture sensor (5 cm depth), and reads will be recorded daily with a Decagon EM50 datalogger.
Canopy multispectral data: A Crop Circle ACS-430 (Holland Scientific Inc, Lincon, NE, USA) optical active sensor will collect canopy reflectance at 670, 730, and 780 nm (red, red-edge, and near-infrared light) in each plot.
A drone DJI Phantom with a Micasense Red-Edge multispectral camera will collect canopy reflectance data at 550, 670, 715, and 840 nm (green, red, red-edge, and near-infrared light) at V3, R2, R6, and R8 soybean stages. Once images are obtained, several vegetation spectral indices (VIs) will be calculated using MATLAB 2023b.
ECa and soil water content: Electromagnetic Induction (EM38) readings (ECa) will be calibrated for EC and soil volumetric water content in the trial area. Each plot will be measured at 0-6 and 6-24 inches in 15-day intervals during the growing season when soil moisture is not under saturation conditions (for example, after rain).
Statistical analysis will be based on an RCBD design with a factorial arrangement. All variables mentioned above will be tested with analysis of variance using the MIXED procedure of SAS 9.4, and the means separation LSD test (P = 0.05).