2025
Increasing soybean yield under drought through enganced symbiotic nitrogen fixation
Contributor/Checkoff:
Category:
Sustainable Production
Keywords:
Abiotic stressAgricultureGeneticsGenomicsNitrogen fixationRhizobiumWater resistanceYield trials
Lead Principal Investigator:
Carrie Miranda, North Dakota State University
Co-Principal Investigators:
Barney Geddes, North Dakota State University
Project Code:
NDSC_2025_Agronomy 3
Contributing Organization (Checkoff):
Institution Funded:
Brief Project Summary:
Drought is a major cause of soybean yield loss. Western North Dakota is characterized by low water or drought-like conditions, with soybean yields suffering as a result. While there may be multiple mechanisms to enhance drought tolerance in soybean, many have a negative effect on agronomic performance and yield during well-watered conditions compared to drought
susceptible varieties. Interestingly, it has been shown that a greater ability to sustain symbiotic nitrogen fixation (SNF) under drought conditions improves soybean yield by 85% (Sinclair et al. 2007 and 2010). The ability to sustain SNF in soybeans has been shown to have a genetic basis, and this trait has been observed in U.S. germplasm collections and is used in some breeding programs. Our long-term aim is to incorporate sustained SNF into NDSU varieties to improve yield in drought conditions. The goal of the project this year is to continue optimizing protocols to test for SNF and apply these protocols to NDSU experimental populations that are segregating for SNF.
Unique Keywords:
#drought, #western north dakota
Information And Results
Project Summary

Drought is considered the number one environmental factor limiting genetic potential of yield (Boyer 1982). In North Dakota, the western part of the state is characterized by low water conditions with an average of 10-16 inches of rain a year compared to the Red River Valley that
receives 20-22 inches of rain a year (nass.usda.gov). Lack of adequate water is one reason why soybean yields average only 30 bushels/acre in the west compared to the Red River Valley that
has soybean yields around 60 bushels/acre (NDSU variety trial data from multiple years). Also,
the need to preserve high yield potential in both well-watered and low-water conditions is fundamental to improving soybean production in the western part of North Dakota. Research has been ongoing for many decades exploring different physiological methods to overcome yield losses due to drought. However, many have proved to be elusive, or plants suffered poor agronomic performance during well-watered conditions (Sinclair et al 2007). Interestingly, research has shown that soybean plants that are able to maintain nitrogen fixation during drought conditions have less yield loss than other susceptible lines (Sinclair et al 2007, 2010). In addition, varieties have been bred that possess the sustained nitrogen fixation trait and still outperform check varieties in well-watered conditions, suggesting that this trait does not have a yield drag associated with it (Chen et al 2007). Although drought research is challenging, focusing on improvement of nitrogen fixation during drought conditions seems to be a
promising direction to explore to improve yields in the driest areas of North Dakota.

Project Objectives

• Objective 1: Optimized screening protocol for determining SNF in soybean germplasm
• Objective 2: Evaluate SNF breeding materials for the SNF trait.

Project Deliverables

• Optimized screening protocol for determining SNF in soybean germplasm. This will allow for a new selection tool for determining which NDSU varieties are most suitable for Western North Dakota based both on yield and the ability to overcome drought
conditions.
• Summer 2024, test SNF breeding lines x NDSU materials for maturity in the field, then test for SNF trait in the greenhouse in winter 2024.

Progress Of Work

Final Project Results

Benefit To Soybean Farmers

Currently, yields in the Western part of ND lag significantly behind those in the Eastern half of the state due to low water conditions. Several farmers in Western ND already prefer NDSU varieties due to the glyphosate tolerance trait and low input cost of seed. This project will allow for screening for sustained nitrogen fixation during drought in newly created elite NDSU
materials. This project will allow the continued incorporation of a drought tolerant trait into NDSU soybean lines targeted to western North Dakota to protect and/or increase yields.

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.