This research aims to 1) to identify potential limitations of nutrient status, mainly nitrogen on yields, 2) to develop strategies to improve protein content, and 3) to improve new soybean varieties with high rates of N fixation for increasing yield. A study conducted by our team already demonstrated that soybeans yields are deaccelerating relative to corn yields. Thus, this research becomes critical to understand the tradeoff between yield and protein and help to move forward not only the productivity of this crop but its sustainability over time. At the end, this project will provide new insights for increasing soybean yields, improve seed quality, reduce environmental impacts and sustainability, and provide greater returns to US farmers.