Root and seedling rot caused by P. sojae is the most important soybean disease in Ohio. Viruses have been by us as tools to examine Rps genes that confer resistance to P. sojae for 10 years. Earlier virus-induced gene silencing (VIGS) vectors have serious shortcomings that limited their wider use. Thanks to the unwavering support from OSC, a superior vector based on cowpea severe mosaic virus, designated FZvec, has been developed. Here we propose to use FZvec to accomplish three goals. First, we will interrogate candidates of two Rps genes, Rps1c and Rps1k, to unveil their true nature. Second, we will attempt to express the P. sojae effectors avrRps1c and avrRps1k to simplify screening of Rps genes. Finally, we will augment P. sojae resistance by enhancing flavonoid production. This project fits OSC mission of investing soybean checkoff funds to maximize the value of soybeans and increase profit opportunities for Ohio farmers, and addresses OSC Strategic Goal of Driving Innovation.