Soybean cyst nematode (SCN), Heterodera glycines, is the most economically important pathogen of soybean in the United States. SCN has spread throughout the major soybean-producing regions of the country and is present in 72 of the 88 Ohio counties. The presence and damage of SCN can go unnoticed while its population densities can increase surpassing damage threshold. Though effective SCN control can be achieved through integrated management practices, we understand less which factors contribute to initial colonization of SCN in fields with no history of infestation. In this work we propose to evaluate how field management history, including weed pressure, influences the initial colonization of soybean cyst nematode on “naïve” fields. We define “naïve” fields as those in which SCN has not been previously detected. Further, we will evaluate how the communities of fungi, present in these soils, and associated to SCN ca influence SCN infection and reproduction.