The proposed research will characterize the phenotypic variation for soybean seed protein and oil, as well as the amino acid profile and approximate fatty acid profile for breeding materials within the soybean breeding program. Typically, in a breeding program, seed protein and oil quantity are measured with a specific Near Infrared Reflectance (NIR) analyzer which can make rapid estimates of protein and oil on samples as small as a few seed. Unfortunately, the NIR in use the soybean breeding program is out of date and calibration is no longer supported. Therefor, each season, our measurements are becoming increasingly irrelevant. Thus, replacing this equipment is an urgent need of the program. Here, we propose to replace the outdated Perkin Elmer DA 7200 NIR with the updated and improved Perkin Elmer DA 7250 NIR analyzer, which would be for use specific to soybean only.