The proposed research will identify and functionally characterize the genes within genetic regions that participate in soybean seed oil and protein production and storage. Ultimately, knowledge of these genes can be used to develop commercial soybean cultivars with improved oil and protein content without diminishing yield.Our specific objectives are to:1) Utilize prior knowledge to identify key genetic regions associated with variation seed oil and protein content and predict the specific genes which are responsible.2) Evaluate the functions of candidate genes in relation to seed oil and protein contents.This project contributes to the larger OSC strategic plan and goals to drive innovations that “support agronomic improvements, technological advancements, and the development of new soybean products”. The results will enhance our knowledge of oil and protein production and storage, enabling breeders to design soybeans with a particular seed composition.