Using soy protein as a fishmeal replacement in domestic aquaculture allows fish producers to decrease operational costs while retaining or enhancing growth performance. There has been tremendous success in integrating soy protein into diets for major aquaculture species (i.e., channel catfish, tilapia, and rainbow trout). There is also an interest in expanding this work to other species using feeds with high levels of fishmeal, such as largemouth bass (LMB). As the demand for this species grows, there is an opportunity to magnify soy protein utilization in this market segment. For bass, there is limited information on the response to soybean process variants and optimized inclusion levels. Type and level of inclusion are integral for optimizing inclusion rates that benefit industry-relevant growth performance and health. The proposed project will evaluate soy protein process variants to investigate LMB growth and development, as well as histological, microbial, and immunological aspects of intestinal health. Together, allowing for the selection of the most appropriate soy ingredient source. The proposed research results are expected to add to the body of knowledge promoting soy-optimized feed formulations and build on existing literature to encourage the use of US-based products in the aquaculture industry.