2022
Selectively Bred, High Protein Soybean Meals for Commercial Production of California Yellowtail
Contributor/Checkoff:
Category:
Export/Trade
Keywords:
Aquaculture
Parent Project:
This is the first year of this project.
Lead Principal Investigator:
Mark Drawbridge, Hubbs-SeaWorld Research Institute
Co-Principal Investigators:
Allen Davis, Auburn University
Project Code:
44209
Contributing Organization (Checkoff):
Institution Funded:
Brief Project Summary:
The California yellowtail represents a high-value fish with an existing market for sushi-grade product. We will test two high protein, selectively bred strains of soybean meal in a laboratory optimization trial of California yellowtail in order to further maximize the inclusion levels of soybean meal in marine fish diets. Feeding trials will be conducted at Hubbs-SeaWorld Research Institute in San Diego, CA. Biochemical composition of the feed and fish will be determined, along with growth and survival metrics, and health assessments.
Key Beneficiaries:
#farmers, #fish farmers
Unique Keywords:
#aquaculture, #california yellowtail, #high protein, #new uses, #soybean meal
Information And Results
Project Summary

In order to “move the needle” on US soy, one is left with but two options: increase the inclusion rate of soy products in existing formulations destined to major commercial species, or develop new markets (“new” species, life stages, or products) where soy can be introduced. This proposal addresses both of these options. First, our test species is the California yellowtail (Seriola dorsalis), which as a member of the genus Seriola, represents a high value species with an existing market for fresh sushi-grade product and successful rearing methods in hatchery and growout. Furthermore, HSWRI is currently involved in efforts to permit a commercial yellowtail farm in federal waters off the coast of southern California. Secondly, our technology partner providing the soybean meal, Benson Hill, is now in commercial production of two high protein soy varieties, including one that has ultra-low oligosaccharide levels to promote acceptability and performance. These varieties need market outlets like those that could be provided my marine finfish aquaculture.
Seafood is responsible for one of the largest natural resource trade deficits in the U.S., exceeding $15 billion and second only to that of oil and gas. There remains a great need for U.S. aquaculture production to fill the domestic seafood void, both in terms of production volume and diversity. Most of this growth is projected to occur with marine fish species, in which a vast research effort for development of plant-based feeds has been, and still is, undertaken. California yellowtail is a highly valued commercial and sport fishes in southern California and is considered an excellent food fish. Farmed yellowtail have previously been provided to commercial growers, primarily in Mexico, as part of demonstration trials. The results from these trials have been very promising and yellowtail are now available as a commercially farmed product for the first time. Great potential exists to expand commercial culture in both northern Baja California, Mexico and Southern California, especially as SoCal was recently announced as one of the first of two Aquaculture Opportunity Areas (AOAs) in the country by NOAA Fisheries. This reinforces the notion that a good opportunity exists right now to demonstrate the effectiveness of diets based on soy protein, coinciding with the developing offshore aquaculture industry in this region. A growing body of research has been developed over recent years, during which standard protocols of reproduction (Stuart and Drawbridge, 2013), larval rearing (Hawkyard et al., 2016; Rotman et al., 2017), and juvenile nutrition (Jirsa et al., 2011; Rombenso et al., 2016 and 2018; Trushenski et al., 2014; Buentillo et al., 2015; Bergman et al., 2018) have been established for yellowtail. Of particular importance, limiting nutrients have been identified: in soy-based formulations, taurine is the first-limiting essential amino acid, followed by methionine (Jirsa et al., 2014). Past research from our group established the taurine requirement for yellowtail at 0.23% (Salze et al., 2015 and 2018). With all of these research results in place, the time is right for a final optimization of one or more soy-based diets, followed by testing in a growout simulation as proposed here.

Proposed Methods/Tactics:
Feeding trials will be conducted at HSWRI’s research facility in San Diego, CA. For the laboratory trial lasting 8 weeks, yellowtail will be fed three different graded formulations of each soy protein variety provided by Benson Hill and a commercial control. Dietary formulations and feed will be provided by Dr. Allen Davis’ laboratory at Auburn University. Each treatment will be replicated three times for statistical validity. Biochemical composition of the feed and fish fillets will be determined at the end of the study, along with growth and survival metrics, and health assessments via necropsy and histopathology.

Project Objectives

We will test two high protein, selectively bred strains of soybean meal in a laboratory optimization trial of California yellowtail in order to further maximize the inclusion levels of soybean meal in marine fish diets.

Project Deliverables

Written reports, feed formulations, presentations, publications.

Progress Of Work

Updated September 1, 2022:

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Updated November 28, 2022:

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Updated February 28, 2023:

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Updated May 19, 2023:

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Final Project Results

Updated August 3, 2023:

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An 8-week feeding trial was successfully conducted using California yellowtail that were feed three different soybean meals (SBMs) at high combined inclusion levels of over 30% for a total of five treatment combinations and a fishmeal control. Yellowtail grew to a maximum average of 141g or 755% weight gain. Survival was high among all treatments (>96%) and food conversion rates were low (1.11-1.16). There were no statistical differences among treatments for any performance measurement. The proximate composition and mineral analysis for the fish at the end of the trial showed no differences between the diets. We also did not see any significant differences in gene expression of intestinal tissue at the end of the trial among five genes evaluated. Qualitative analysis of the intestinal tissues via histology yielded no significant differences across the dietary treatments.

This study showed that fish performance was not impacted by different SBMs or different inclusion rates used in this study. In fact, performance from those diets was similar to the FM control, which bodes well for future commercial application. Further refinements will need to be made to include more SBM in the diets. Our study suggested that it is possible that larger fish could have performed well on diets with no fish meal – diets that smaller fish rejected. Larger fish would be the target for growout trials where most of the feed biomass is consumed, so this area should be explored in future research.

Benefit To Soybean Farmers

Expanded markets for soybean meal

The United Soybean Research Retention policy will display final reports with the project once completed but working files will be purged after three years. And financial information after seven years. All pertinent information is in the final report or if you want more information, please contact the project lead at your state soybean organization or principal investigator listed on the project.